Progress 09/01/09 to 08/31/10
OUTPUTS: Uterine samples from six gilts were obtained from a local slaughterhouse. These tissues were utilized to determine the efficacy of a "safe" fixative solution (to try to avoid the utilization of paraformaldehyde). Also tested were procedure conditions for tissue-embedding in paraffin. Two workshops, one for students of a special topic course, and one for high school students (12th grade), were offered for teaching tissue processing for histological and immunohistochemical studies. Also the new equipment, a bright light microscope with a research quality digital camera, and a computer were acquired. Also purchased was a high capacity tank for storage of samples in liquid nitrogen. Many of the reagents and laboratory consumables have been acquired in preparation for tissue collection and for other laboratory procedures related to this investigation (., real time pcr plates, tips, gloves, tissue cassettes for histological processing, paraffin). PARTICIPANTS: Project leader, Esbal Jimenez, ., is in charge of grant and project management. He is in charge of requesting quotes and submitting orders for materials, reagents, equipment and any other necessity for the investigation. He also trains students regarding laboratory safety and molecular biology techniques. An undergraduate student (Victor Ramos, future . graduate student) was trained in tissue processing for histological and immunohistochemical studies. This student was also trained to utilize photo editing/analysis software (ImageJ) for publication and for determining color intensity (for immunohistochemistry results). TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Not relevant to this project.
Optimal conditions for tissue fixation were determined and will be reported in the final scientific manuscript resulting from this research. Unfortunately, the "safe" fixative solution did not perform as well as manufacture stated; therefore, we will use 4% paraformaldehyde in our continuing research.
nuclear hormone receptors superfamily are a wide group of proteins which function is to act as transcription factors in order to modulate in a positive or negative way the expression of genes involved in differentiation processes, growth, reproduction and metabolism. given its participation in key pathologic processes, the disfunctions associated to these receptors have huge implications in diseases of great importance in public health such as cardiovascular disease, diabetes mellitus type 2, and cancer between others. some aspects of this protein superfamily are reviewed in this study, including its structure, relationship with lipid metabolism and cardiovascular implications. this study focuses on the peroxisome proliferator-activated receptor (ppar), and briefly on the liver x receptors (lxr).